Adobe White Rabbit Photoshop Cs5 ((LINK))
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Methods : Under general and topical anesthesia with tetracaine hydrochloride, six 3 month-old white New Zealand rabbits received subconjuntival injection of 20,000 murine fibroblast cells (NIH 3T3 cell line) and 5 µL of Matrigel(Corning Inc, NY, US) on the perilimbal temporal bulbar conjunctiva of the right eye using a 1ml 30G needle. Left eyes were injected with 5 µL of Matrigel under the perilimbal temporal bulbar conjunctiva (control). Eyes were photographed under a magnification of 16x using a 12 megapixel digital camera attached to the microscope (WPI, Inc. FL, US) on day 1, 3 and 7. Conjunctival vascularization was measured on day 1,3 and 7 by analyzing images using Adobe PhotoshopCS5 (Adobe Inc,San Jose,CA) color histograms to measure red pixel saturation on a 6x6 mm area on the site of injection. Area of corneal and conjunctival fibrovascular tissue formation was assessed by analyzing the images on day 3 and 7 using area measurement software (Adobe Photoshop CS5). Statistical analysis was performed using t-test for mean comparison, statistical significance was considered with a p value
Conclusions : Subconjunctival injection of murine fibroblast cells and Matrigel in white New Zealand rabbits was more effective for inducing conjunctival vascularization and fibrovascular tissue growth on the site of injection and surrounding cornea than subconjunctival Matrigel alone. A novel animal model for pterygium is presented with potential use for research on molecular mechanisms of pathogenesis and new treatment strategies.
- Hey gang, this is Deke McClelland. Welcome to Deke's Techniques. Now, on previous weeks, we've been exploring how to paint abstract art, in particular in the style of Spanish surrealist, Joan Miro. Now, if you know anything about Miro, and who doesn't, you know that he dabbled in Fauvism and Cubism before ultimately developing his own visual vocabulary. So, I thought we'd spend some more, additional time, exploring such pieces as, for example, "The Sun" or "El Sol," in this case, as redrawn by me. But it's just such an awesome piece. But then al